EpiCypher 2023: Biological and Clinical Frontiers in Epigenetics

Visit us at booth #NA

Learn about our new 5-letter sequencing technology – duet multiomics solution +modC – and discover the combinatorial power of genetics and epigenetics.

5 November 2023
to 10 November 2023

Grand Fiesta Americana Coral Beach Cancún, Mexico, Cancun

About the event

This exciting conference will offer the opportunity to interact with leading experts in the chromatin and epigenetics fields and will foster the exchange of scientific ideas between meeting participants in a relaxing and casual environment. The meeting will blend talks from invited speakers from academia and industry with many talks selected from submitted abstracts.

Discriminating 5mC and 5hmC at single-base resolution

Aldo Ciau-Uitz

Senior Scientist

biomodal

11.6.23 / 12:20PM – 12:40PM

Presentation abstract

DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding of biology. In human genomes, an epigenetic modification at the fifth carbon of cytosines bases comprises one fundamental pathway by which genes canbe silenced or activated. Methods widely used to detect epigenetic modifications at cytosinebases rely on either deamination of unmodified cytosines to read as thymine or borane reduction of the modified oxidised cytosine bases to read as thymine. As a result, such methods fail to capture common C-to-T mutations, and importantly also fail to distinguish 5-methylcytosine (5mC – mainly found in silenced parts of the genome) from 5-hydroxymethylcytosine (5hmC – enriched in active gene bodies and enhancers). Hence, existing methods are unable to read the complete information stored in our genomes in a single workflow.

Here, we build on our newly reported duet multiomics solution +modC, which has the unique ability to read genetic and epigenetic cytosine modifications (modC) in a single workflow. Byintroducing an important high-fidelity methyltransferase during the workflow, we are able to construct an expanded information table that can be used to deconvolute A,T,C,G, 5mC and5-hmC simultaneously in a single read within a DNA molecule. Using synthetic controls we demonstrate the performance of this technology and report on error rates associated with the method. Finally, we use a mouse embryonic stem cell-line, ES-E14TG2A, to map for the first time a simultaneous reading of the genome and epigenome at high depth and show how these epigenetic modifications are segregated across the genome.

In summary, using our modified approach we demonstrate simultaneous, phased reading of all six genetic and epigenetic bases. This tool provides a more complete picture of the information stored in genomes and has applications throughout biology and medicine.

Presentation abstract

One sample. One workflow. One solution.

Here are the relevant biomodal resources for information. Find poster presentation information, case studies, interviews, and more.

Related resources

Refining liquid biopsy: generating more information from cell free DNA

Refining liquid biopsy: generating more information from cell-free DNA

Learn how duet multiomics solution +modC is transforming research efforts in early cancer detection, risk stratification, and disease monitoring by using liquid biopsy for profiling of cfDNA. This poster illustrates how capturing complete genetic and epigenetic information in one workflow, from a single low-volume DNA sample is more powerful for early disease detection than either alone.

Attending from biomodal

Director of Sales and Business Development for North America
EpiCypher 2023

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