Integrated genetics and methylation, from the same sample.
duet +modC measures genetic variants and modified cytosine together from a single sample, across gDNA, FFPE and cfDNA, with market-leading sensitivity, specificity and genetic accuracy.
Accurate genetics and high-quality methylation from one workflow
Genetic variation and DNA methylation work together to shape biological function, disease development and cellular state. Yet many workflows require separate assays, additional sample material and downstream integration of independent datasets.
duet +modC brings genetic variants and modified cytosine (modC) together from the same DNA molecule in a single workflow. duet's hairpin architecture copies and retains the original genetic sequence before conversion, so you keep confident variant detection while measuring high-quality methylation from the same sample.
The result is integrated genetic and epigenetic data that supports confident biological interpretation while simplifying experimental design and analysis, when independent 5mC and 5hmC resolution is not required.
Why choose duet +modC?
Genetics and methylation, together
5 bases from a single molecule, integrated in one workflow and one dataset rather than combined from separate assays downstream.
High-quality methylation: sensitivity and specificity
Measure modified cytosine with market-leading sensitivity and specificity, reducing the false-positive calls and missed events that obscure biological signal.
High-confidence genetic variant detection
duet's hairpin approach copies and retains the original genetic sequence before conversion, delivering confident C>T variant detection, including low-frequency variants, while resolving methylation from the same molecule.
One workflow, every sample type
Generate integrated genetic and methylation data from gDNA, FFPE and cfDNA using a single workflow, rather than maintaining separate assays for different sample types.
No harsh chemistry
duet uses a gentle enzymatic workflow, with no bisulfite and no harsh chemical conversion that degrades and destroys DNA before you can read it.
Built-in error correction
Because duet reads both the original and copy strands, its read-resolution software flags implausible strand pairings as an N, filtering out sequencing and PCR errors that traditional methods carry through.
Performance data
High sensitivity and specificity for modified cytosine detection
duet +modC detects modified cytosine with high sensitivity and specificity, helping researchers identify more true methylation events while reducing false-positive calls and missed signals.
Methylation sensitivity and specificity of duet 6-base mosaic evaluated using the included controls and compared to published or internally generated data for other epigenetic sequencing technologies. duet 6-base mosaic has higher sensitivity and a 25-fold lower false positive rate than company I.
High-confidence genetic variant detection
duet +modC preserves accurate variant detection while simultaneously generating modified cytosine data, including confident detection of biologically important C>T mutations, with SNP calling accuracy comparable to leading genetic sequencing approaches.
Germline variant calling performance using NA12878 at 30x coverage
Software included, analysis on your terms
Every duet +modC kit includes the duet software pipeline and modality XPLR, taking you from raw reads to integrated genetics and methylation without assembling your own pipeline. Run it on a workstation, your HPC or the cloud, and explore multiomic results with no dedicated bioinformatician required. Analysis tools come standard, and your data stays yours.
Applications
duet +modC supports research that benefits from integrated genetic and epigenetic analysis across sample types, without requiring independent 5mC and 5hmC resolution.
- Aging studies
- Allele-specific methylation analysis
- Biomarker discovery
- Cancer research
- Differential methylation analysis
- Epigenotyping
- Fragmentomics
- Liquid biopsy (cfDNA/ctDNA) studies
- Minimal residual disease (MRD) assay development
- Multi-cancer early detection (MCED) assay development
- Population studies, including epigenome-wide association studies (EWAS)
- Simultaneous detection of genetic variants and methylation
- Tumor profiling, including FFPE
- Whole-genome or targeted methylation sequencing
Ordering information
Request quote| Catalog number | Product name | Product description |
|---|---|---|
| 5205 | duet +modC 8x reaction | duet +modC assay, duet software, modality XPLR for pre and post-sequencing workflows for 8 reactions |
| 5206 | duet +modC 24x reaction | duet +modC assay, duet software, modality XPLR for pre and post-sequencing workflows for 24 reactions |
| 4103 | UDI 8x reactions | Unique dual indices for 8 reactions |
| 4102 | UDI 24x reactions | Unique dual indices for 24 reactions |
| 4104 | UDI 96x reactions | Unique dual indices for 96 reactions |
| Specifications | |
|---|---|
| Sample types | gDNA, FFPE, cfDNA |
| Input requirements | 10–80 ng gDNA; 5–30 ng cfDNA |
| Sequencing compatibility | Standard short-read sequencing platforms |
| Analysis | duet software + modality XPLR |
Software outputs • Resolved FASTQ • BAM • VCF (germline variant calls) • QC reports • Allele specific methylation file • Zarr datastore (modC data)