duet multiomics solution +modC

duet multiomics solution

Discover the full story of genetic and epigenetic change

Measure all four genetic bases (A-C-G-T) without ambiguity in C or T calls – plus modified cytosine (modC) – using your existing next-generation sequencing pipeline, phased within a single read.

Reveal more genetic data, all at once

The combination of genetic and epigenetic modifications such as 5mC plays a pivotal in understanding the human genome. The duet multiomics solution +modC enables you to capture both simultaneously within a single DNA sample, in a single workflow – providing powerful insights on the dynamic interactions that are occurring.

duet multiomics solution +modC workflow

What is duet multiomics solution +modC?

duet multiomics solution +modC combines the duet assay and duet software to investigate genetic sequence and modified cytosine simultaneously.

duet +modC assay

The duet +modC library preparation kit takes a low input DNA sample (10ng) and copies each DNA strand across a hairpin structure. The original strand maintains any modified cytosines, and is used for epigenetic analysis, and the copy strand is used for genetic sequence analysis.

duet assay hairpin

duet software

The duet software then takes the sequence information from pairs of original and copy strands and aligns them. For each position, it compares both original and copy strands to compute a resolved base that has complete genetic and epigenetic information.

From there, use our comprehensive data analysis package to explore methylation profiles, genetic variants, and variant-associated methylation in your samples, and compare epigenetic changes across genetic windows or contexts, in a single sample or within multi-sample cohorts.

Read resolution

Product contents

Unit
Product numbers
duet multiomics solution +modC 8 reaction kit
duet multiomics solution +modC contains:
  • duet assay: a pre-sequencing workflow with hairpin technology, comprised of 2 boxes containing all the necessary reagents to create 8 sequencer-ready libraries with ligated Illumina adaptors.
  • duet software: a post-sequencing analysis software module, which can be deployed on premises or on the cloud, and provides phased genetic variant calls plus modified C at read level in VCF, Quant and BED Methyl formats.
5103
duet multiomics solution +modC 24 reaction kit
duet multiomics solution +modC contains:
  • duet assay: a pre-sequencing workflow with hairpin technology, comprised of 2 boxes containing all the necessary reagents to create 24 sequencer-ready libraries with ligated Illumina adaptors.
  • duet software: a post-sequencing analysis software module, which can be deployed on premises or on the cloud, and provides phased genetic variant calls plus modified C at read level in VCF, Quant and BED Methyl formats.
5104
duet UDIs 1-8 reaction kit
4101
duet UDIs 1-24 reaction kit
4102
duet UDIs 1-96 reaction kit
4104

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Cambridge Epigenetix is now biomodal