Discover how the duet technology captures genetic and epigenetic information from the same DNA molecule.
Most methylation sequencing methods rely on conversion, which alters the original DNA sequence and can make it difficult to distinguish true genetic variants from methylation-derived changes.
duet overcomes this challenge with a proprietary hairpin approach. Before conversion, each DNA strand is copied from a hairpin adapter, preserving the original genetic sequence. The original and copied strands are then sequenced together as a linked molecule.
duet software compares both strands to reconstruct the original DNA sequence while identifying modified cytosines (duet +modC and duet +modC mosaic) or distinguishing 5mC and 5hmC (duet evoC and duet 6-base mosaic).
By analyzing both strands together, duet accurately calls variants, resolves epigenetic state, and suppresses PCR and sequencing errors.
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While the duet hairpin workflow preserves genetic and epigenetic information from high-quality DNA, cfDNA presents a different challenge. These samples are highly fragmented, damaged, and often available in limited amounts. Conventional library preparation workflows rely on end repair and A tailing, which can lose short or damaged molecules and alter original DNA features. duet mosaic was developed to address this challenge, using single-stranded ligation to recover more cfDNA molecules, including those that are single stranded, while preserving native fragment structure and base modifications for downstream analysis.
duet mosaic’s single stranded ligation preserves original cfDNA
features for lower LoD and better biomarker discovery
Researchers shouldn't have to choose between a complete biological picture and a practical workflow. By generating genetic, epigenetic, and fragmentomic information from the same sample in a single workflow, duet eliminates the need for multiple assays and fragmented datasets. This streamlined approach reduces complexity, conserves sample material, and delivers richer insights from every experiment.
Generate genetic and epigenetic insights from the same sample without compromising accuracy, biological insight or workflow simplicity.
Explore the duetOur comprehensive software and data analysis package allows you to easily identify methylation profiles, genetic variants, and variant-associated methylation in a single sample or multi-sample cohort.
Explore duetmodality XPLR is an accessible and scalable software tool designed to uncover disease-relevant signatures, link methylation to gene regulation, and support biomarker discovery—empowering you to identify, classify, and monitor disease.
Explore modalityWhether you're studying disease biology, identifying biomarkers, investigating gene regulation, or developing liquid biopsy assays, the duet platform generates integrated genetic and epigenetic information designed to help researchers uncover meaningful biology with confidence.