Resolve genetics, and complete epigenetics on the same molecule.
duet evoC delivers genetics, 5mC and 5hmC as distinct signals from a single sample, across gDNA, FFPE and cfDNA, revealing biology that combined-readout methods miss.
Accurate genetic and complete epigenetic insights from the same sample
Disease biology is shaped by both genetic variation and epigenetic regulation. Resolving genetics, 5mC and 5hmC independently, provides a higher-definition view of biological function and change.
duet evoC is designed to bring those pieces together. duet's hairpin architecture preserves confident genetic variant detection and independently resolves 5mC and 5hmC from the same molecules, in a single workflow, across gDNA, FFPE and cfDNA.
The result is integrated genetic and complete epigenetic insight from one sample, without the compromises of running separate assays or the need to combine multiple 5mC and 5hmC workflows into a single measurement.
Why choose duet evoC?
Genetics, 5mC and 5hmC, resolved
6 bases from a single molecule, each able to reveal biology on its own, and far more powerful together, with 5mC and 5hmC read independently rather than combined.
High-quality sensitivity and specificity
Read 5mC and 5hmC as distinct signals, each at high sensitivity and specificity, revealing powerful biomarkers and regulatory biology that other methods cannot separate.
High-confidence genetic variant detection
duet's hairpin approach copies and retains the original genetic sequence before conversion, delivering confident C>T variant detection, including low-frequency variants, while resolving methylation from the same molecule.
One workflow, every sample type
Generate integrated multiomic data from gDNA, FFPE and cfDNA using a single workflow, rather than maintaining separate assays for different sample types.
No harsh chemistry
duet uses a gentle enzymatic workflow, with no bisulfite and no harsh chemical conversion that degrades and destroys DNA before you can read it.
Built-in error correction
Because duet reads both the original and copy strands, its read-resolution software flags implausible strand pairings as an N, filtering out sequencing and PCR errors that traditional methods carry through.
Performance data
Resolve 5mC and 5hmC and unmodified cytosine independently
duet evoC resolves all 3 states of cytosine independently, distinguishing biologically distinct signals that other approaches combine into one measurement. Combining them into a single modC readout can hide real regulatory biology.
The genome-wide percentage of cytosine states at CpGs in cerebellum tissue, independently reported by duet evoC as unmodified cytosine (C), 5mC and 5hmC.
High-quality methylation: sensitivity and specificity
High sensitivity and specificity reduce false-positive methylation calls and missed events, helping researchers focus on real biological signal rather than analytical noise.
Methylation sensitivity and specificity of duet 6-base mosaic evaluated using the included controls and compared to published or internally generated data for other epigenetic sequencing technologies. duet 6-base mosaic has higher sensitivity and a 9-fold lower false positive rate than company I.
High-confidence genetic variant detection
duet evoC delivers SNP calling accuracy comparable to leading genetic sequencing approaches while simultaneously generating epigenetic data, including confident detection of C>T mutations.
Germline variant calling performance when sequencing NA12878 at 30x coverage
Software included, analysis on your terms
Every duet evoC kit includes the duet software pipeline and modality XPLR, taking you from raw reads to resolved genetics and methylation without assembling your own pipeline. Run it on a workstation, your HPC or the cloud, and explore multiomic results with no dedicated bioinformatician required. Analysis tools come standard, and your data stays yours.
Applications
duet evoC supports research requiring integrated genetic and epigenetic analysis across sample types.
- Aging studies
- Allele-specific methylation analysis
- Biomarker discovery
- Cancer research
- Differential methylation analysis
- Epigenotyping
- Fragmentomics
- Liquid biopsy (cfDNA/ctDNA) studies
- Minimal residual disease (MRD) assay development
- Multi-cancer early detection (MCED) assay development
- Neurodegenerative research
- Neuroscience and developmental biology
- Population studies, including epigenome-wide association studies (EWAS)
- Simultaneous detection of genetic variants and methylation
- Tumor profiling, including FFPE
- Whole-genome or targeted methylation sequencing
Publications
Researchers are using duet evoC across cancer, immunology and epigenetics. Selected publications:
Ordering information
Request quote| Catalog number | Product name | Product description |
|---|---|---|
| 6205 | duet evoC 8x reaction | duet evoC assay, duet software, modality XPLR for pre and post-sequencing workflows for 8 reactions |
| 6206 | duet evoC 24x reaction | duet evoC assay, duet software, modality XPLR for pre and post-sequencing workflows for 24 reactions |
| 4103 | UDI 8x reactions | Unique dual indices for 8 reactions |
| 4102 | UDI 24x reactions | Unique dual indices for 24 reactions |
| 4104 | UDI 96x reactions | Unique dual indices for 96 reactions |
| Specifications | |
|---|---|
| Sample types | gDNA, FFPE, cfDNA |
| Input requirements | 10–80 ng gDNA; 5–30 ng cfDNA |
| Sequencing compatibility | Standard short-read sequencing platforms |
| Analysis | duet software + modality XPLR |
Software outputs • Resolved FASTQ • 6-base BAM • VCF (germline variant calls) • QC reports • Allele specific methylation file • Zarr datastore (6-base methylation)