Epigenetic Society Meeting of Rome

Visit us at booth #NA

Explore biology in every mode.

Capture genetic and epigenetic information simultaneously in a single workflow to better understand normal and disease biology.

12 October 2023
to 14 October 2023

Conference Center “Roma Eventi-Fontana di Trevi”, Rome

About the event

The 1st Epigenetics Society International Meeting will be held on Oct. 12 -14 in a conference center in the heart of Rome’s tourist attractions. We have an extraordinary array of speakers (5 Keynote speakers and 19 Featured Speakers) covering a broad array of topics in basic, clinical, and translational epigenetics.

Discriminating 5mC and 5hmC at single-base resolution

Minna Taipale

Staff Scientist

biomodal

Friday, 13 October @ 5:35pm

Presentation abstract

DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding of biology. In humans, an epigenetic modification at the cytosines comprises one fundamental pathway by which genes are regulated. Methods widely used to detect epigenetic modifications at cytosine rely on either deamination of unmodified cytosines to read as thymine or borane reduction of the modified oxidised cytosine bases to read as thymine. As a result, such methods fail to capture common C-to-T mutations, and also fail to distinguish 5-methylcytosine from 5-hydroxymethylcytosine. Hence, existing methods are unable to read the complete information stored in a single workflow. Here, we build on our newly reported duet multiomics solution +modC, which has the unique ability to read genetic and epigenetic cytosine modifications (modC) in a single workflow. By introducing an important high-fidelity methyltransferase during the workflow, we are able to deconvolute A,T,C,G, 5-mC and 5-hmC simultaneously in a single read within a DNA molecule. Using synthetic controls we demonstrate the performance of this technology and report on associated error rates. Finally, we use a mouse embryonic stem cell-line, ES-E14TG2A, to map for the first time a simultaneous reading of the genome and epigenome at high depth and show how these epigenetic modifications are segregated across the genome. In summary, using our modified approach we demonstrate simultaneous, phased reading of all six genetic and epigenetic bases. This tool provides a more complete picture of the information stored in genomes and has applications throughout biology and medicine.

One sample. One workflow. One solution.

Here are the relevant biomodal resources for information. Find poster presentation information, case studies, interviews, and more.

Related resources

Refining liquid biopsy: generating more information from cell free DNA

Refining liquid biopsy: generating more information from cell-free DNA

Learn how duet multiomics solution +modC is transforming research efforts in early cancer detection, risk stratification, and disease monitoring by using liquid biopsy for profiling of cfDNA. This poster illustrates how capturing complete genetic and epigenetic information in one workflow, from a single low-volume DNA sample is more powerful for early disease detection than either alone.

Attending from biomodal

Director of Sales and Business Development, Europe
Epigenetic Society Mtg Rome Oct 2023

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