How are you able to detect epigenetic modifications in my sample?

Our duet multiomics solution evoC protects cytosines that display epigenetic markers during library preparation without degrading your sample. 5mC markers are copied from the original DNA strand to a complimentary synthesized strand attached with a hairpin sequence, whilst 5hmC markers remain only on the original strand. Unmodified cytosines on both strands are then enzymatically deaminated. The duet bioinformatics pipeline uses base information from both strands to decode the original base at each position along with epigenetic marks highlighted as the fifth and sixth genetic letter, resulting in a 6-base genome. For more information on how this occurs, read our paper here.