The duet pipeline can seamlessly perform read resolution, alignment, variant calling, summarisation of methylation status and produce reports and QC information. Here is what you can expect in terms of processing times:
- Running in a cloud tenancy, allocating exactly the compute resources required at each stage: ~30 hours
- Running on a single dedicated host with 32 cores and 64 GB RAM: 11-12 days
- Using a Slurm cluster of 4 hosts, each with 32 cores and 64 GB RAM: 3-4 days
The above function as a guideline to give an indication of potential elapsed pipeline runtime and is highly dependent upon the setup of your system. The timings have been calculated assuming 8 samples and approximately 30X coverage.
Have questions or need assistance with your system setup? We’re here to help! Feel free to reach out to us for further discussions.