Cold Spring Harbor Laboratory – 2024 Epigenetics & Chromatin

Join us for a dynamic workshop on DAY 3 of the conference! Register at the bottom of the page and join us for our workshop on Thursday, September 19, 2024, in the Hershey Building (Upstairs), following the morning session and lunch will be provided. The workshop will be hosted by biomodal, featuring Lindsey Guerin from Vanderbilt University. Please note that the workshop is free, but registration is recommended.  
When: Thursday, September 19th, 2024   Time: Following morning sessions (lunch will be provided)  Location: Hershey Building (Upstairs) 
17 September 2024
to 21 September 2024
Cold Spring Harbor Laboratory
, Cold Spring Harbor

About the event

You are cordially invited to participate in the seventh Cold Spring Harbor Laboratory meeting on Epigenetics and chromatin. The opening session will begin at 7:30 p.m. on Tuesday, September 17 and the meeting will end after lunch on Saturday, September 21, 2024. As currently planned, the program will have eight sessions devoted to oral presentations, together with three poster sessions for presentations that cannot be accommodated in the formal sessions. Selection of talks for the oral sessions will be made by the organizers in conjunction with the session chairpersons, from the submitted abstracts.

Keynote Speakers:
Asifa Akhtar, MPI of Immunobiology and Epigenetics, Germany
Kristian Helin,
 Institute of Cancer Research, UK

Topics:

  • Histone Modifications
  • Epigenetic Memory
  • Chromatin Structure & Remodeling
  • Chromatin Architecture & Nuclear Organization
  • Gene Expression
  • Epigenetic & Genetic Stability
  • Epigenetics in Disease
  • Epigenetics in Development

Presenting at the event

Temporally discordant chromatin accessibility and DNA demethylation define short and long-term enhancer regulation during cell fate specification

Lindsey Guerin

Graduate Student

Vanderbilt University

Thursday, September 19th, 2024 | Following the morning session and lunch will be provided

Presentation abstract

Epigenetic mechanisms govern the transcriptional activity of lineage-specifying enhancers; but recent work challenges the dogma that joint chromatin accessibility and DNA demethylation are prerequisites for transcription. To understand this paradox, we established a highly-resolvedtimeline of DNA demethylation, chromatin accessibility, and transcription factor occupancy during neural progenitor cell differentiation. We show thousands of enhancers undergo rapid, transient accessibility changes associated with distinct periods of transcription factor expression. However, most DNA methylation changes are unidirectional and delayed relative tochromatin dynamics, creating transiently discordant epigenetic states. Genome-wide detection of 5-hydroxymethylcytosine further revealed active demethylation begins ahead of chromatin and transcription factor activity, while enhancer hypomethylation persists long after these activities have dissipated. We demonstrate that these timepoint specific methylation states predict past, present and future chromatin accessibility using machine learning models. Thus, chromatin and DNA methylation collaborate on different timescales to mediate short and long-term enhancer regulation during cell fate specification

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