Festival of Genomics & Biodata-London 2024

During this session, you will learn how to;

• Preserve Valuable Samples: Utilise samples as low as 10 ng of DNA for highly accurate modC.
• C-to-T Mutation Detection: Simultaneously detect the most common type of genetic mutation (C-to-T) in the human genome and cancer.  
• Whole Genome Investigation: Get simultaneous whole genome sequencing results with standard SNP detection and epigenetic marks with improved sensitivity and specificity.  
• Allele-specific methylation: Attain phased results for allele-specific methylation (ASM) and maintain even genomic coverage.

The reprogramming process is capable of reversing multiple hallmarks of ageing, however complete reprogramming to iPSCs does not appear to be required for this as molecular features such as the epigenome are already rejuvenated by the maturation phase of iPSC reprogramming. We have developed a method called “maturation phase transient reprogramming”, where the factors required for reprogramming (OSKM) are expressed until this rejuvenation point and then withdrawn.

Applying MPTR to dermal fibroblasts from middle age donors, cells temporarily lose and then reacquire their fibroblast identity. Excitingly, this process substantially rejuvenates multiple cellular attributes including transcriptomic and DNA methylation ageing clocks. We are now investigating the effect of MPTR on the genome and epigenome in more detail and have performed 5-letter sequencing on fibroblasts after MPTR. We have found that transient reprogramming does not promote further mutations compared to cells that are not reprogrammed. In addition, 5L sequencing has enabled us to examine allele-specific methylation, which we found to be particularly increased on the X chromosome after MPTR. This sequencing technology is enabling us to better understand the safety of MPTR as well as the mechanisms that may be responsible for rejuvenation.