duet multiomics solution +modC

Gain complete genetic and epigenetic methylation information from your DNA library in a single workflow. All four genetic bases (A-C-G-T) without ambiguity in C or T calls – plus modified cytosine (modC) using your existing next-generation sequencing pipeline, phased within a single read.

duet multiomics solution +modC

Reveal more genetic data, all at once

High accuracy reads

The duet multiomic solution inherently suppresses PCR and sequencing errors with a base call accuracy in excess of Q40.

Seamless integration

Supercharge your genetic insights without costly in-house bioinformatics or changing your current sequencing platform.

Optimised bioinformatics

Precise sequence information in one run, eliminating the inaccuracy of inferred methylation as well as data loss from merged sequencing workflows.

Download the specification sheet
Download the workflow in PPTX format

Discover the full story of genetic and epigenetic change

Multichannel pipette

Variant-associated methylation

Acquiring genetic and methylation data simultaneously produces higher quality calls that show how methylation promotes or silences specific genes causing deregulation, altered expression or potential disease.

Scientist using a pipette

Liquid biopsy

Maximise the genetic data you obtain from cell-free DNA (cfDNA) with our combined workflow, allowing you to use precious low input DNA samples to detect and monitor disease states.

Scientists examining results

Whole genome sequencing

Don’t miss crucial information as you investigate the genetic and methylation states of the entire human genome.

FAQs

  • Why does seeing methylation matter?
    The four traditional bases make up the genetic code which is a vital foundation for all living systems. But DNA methylation affects how and when genes are expressed. Capturing both genetic and epigenetic information is essential to understanding normal biology and disease states, as well as developing precision therapies.
  • Will this solution require changes to our existing sequencing pipeline?
    No, the duet multiomic solution +modC integrates seamlessly with your current Illumina sequencer.
  • How long does the duet multiomic solution +modC workflow take?
    Our workflow is similar to other standard library preparation and sequencing workflows, with an assay turnaround time of less than 16 hours and an assay hands on time of less than 8 hours.
  • How are you able to detect epigenetic modifications in my sample?
    Our duet multiomics solution +modC protects cytosines that display epigenetic markers during library preparation without degrading your sample, and our bioinformatics pipeline supresses sequencing errors and furthermore highlights modified cytosines as a fifth genetic letter. For more information on how this occurs, read our paper here.
  • What's in the box?
    duet multiomics solution +modC contains:
    •  a pre-sequencing workflow, comprised of 2 boxes containing all the necessary reagents to create 8 or 24 sequencer-ready libraries with ligated Illumina adaptors.
    • a post-sequencing analysis software module, which can be deployed on premises or on the cloud, and provides phased genetic variant calls plus modified C at read level in VCF, Quant and BED Methyl formats.

Tell us what you need

Find out how our solution can help you gain a deeper understanding of biology

Download the duet multiomics +modC sample data

Cambridge Epigenetix is now biomodal