duet multiomics solution +modC
Discover the full story of genetic and epigenetic change
Measure all four genetic bases (A-C-G-T) without ambiguity in C or T calls – plus modified cytosine (modC) – using your existing next-generation sequencing pipeline, phased within a single read.
Reveal more genetic data, all at once
The combination of genetic and epigenetic modifications such as 5mC plays a pivotal in understanding the human genome. The duet multiomics solution +modC enables you to capture both simultaneously within a single DNA sample, in a single workflow – providing powerful insights on the dynamic interactions that are occurring.
What is duet multiomics solution +modC?
duet multiomics solution +modC combines the duet assay and duet software to investigate genetic sequence and modified cytosine simultaneously.
duet +modC assay
The duet +modC library preparation kit takes a low input DNA sample (10ng) and copies each DNA strand across a hairpin structure. The original strand maintains any modified cytosines, and is used for epigenetic analysis, and the copy strand is used for genetic sequence analysis.
duet software
The duet software then takes the sequence information from pairs of original and copy strands and aligns them. For each position, it compares both original and copy strands to compute a resolved base that has complete genetic and epigenetic information.
From there, use our comprehensive data analysis package to explore methylation profiles, genetic variants, and variant-associated methylation in your samples, and compare epigenetic changes across genetic windows or contexts, in a single sample or within multi-sample cohorts.
Product contents
Unit | Product numbers |
|---|---|
duet multiomics solution +modC 8 reaction kitduet multiomics solution +modC contains:
| 5103 |
duet multiomics solution +modC 24 reaction kitduet multiomics solution +modC contains:
| 5104 |
duet UDIs 1-8 reaction kit | 4101 |
duet UDIs 1-24 reaction kit | 4102 |
duet UDIs 1-96 reaction kit | 4104 |
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Find out how our solution can help you gain a deeper understanding of biology
FAQs
How are you able to detect epigenetic modifications in my sample?
Our duet multiomics solution +modC protects cytosines that display epigenetic markers during library preparation without degrading your sample, and our bioinformatics pipeline supresses sequencing errors and furthermore highlights modified cytosines as a fifth genetic letter. For more information on how this occurs, read our paper here.
Will this solution require changes to our existing sequencing pipeline?
No, the duet multiomic solution +modC integrates seamlessly with your current Illumina sequencer.
How long does the duet multiomic solution +modC workflow take?
Our workflow is similar to other standard library preparation and sequencing workflows, with an assay turnaround time of less than 16 hours and an assay hands on time of less than 8 hours.
Why does seeing methylation matter?
The four traditional bases make up the genetic code which is a vital foundation for all living systems. But DNA methylation affects how and when genes are expressed. Capturing both genetic and epigenetic information is essential to understanding normal biology and disease states, as well as developing precision therapies.
