The integration of genetic and epigenetic information is essential for a comprehensive understanding of genome function and regulation. Traditional sequencing methods often fall short in capturing both genetic variants and epigenetic modifications such as 5‑methylcytosine (5mC) and 5‑hydroxymethylcytosine (5hmC) simultaneously. Recent advances in 6-base sequencing have enabled the simultaneous, base-resolution detection of canonical bases and key cytosine modifications in a single workflow. This review explores the biological significance of 5mC and 5hmC, discusses current methods to achieve 6-base sequencing, and highlights recent applications in academic and clinical settings.
For Methylation Sequencing, The Cancer Is In The Details
5 March 2026
Learn how 6‑base sequencing, which separates 5mC and 5hmC detection, reveals subtle early epigenetic changes that dramatically improve stage I colorectal cancer detection compared with traditional methylation methods.
