The integration of genetic and epigenetic information is essential for a comprehensive understanding of genome function and regulation. Traditional sequencing methods often fall short in capturing both genetic variants and epigenetic modifications such as 5‑methylcytosine (5mC) and 5‑hydroxymethylcytosine (5hmC) simultaneously. Recent advances in 6-base sequencing have enabled the simultaneous, base-resolution detection of canonical bases and key cytosine modifications in a single workflow. This review explores the biological significance of 5mC and 5hmC, discusses current methods to achieve 6-base sequencing, and highlights recent applications in academic and clinical settings.
6-base multi-modal data is a powerful liquid biopsy platform for cancer detection, enabling the discovery of epigenetic, genetic and fragmentomics biomarkers in a single workflow
17 June 2026
We evaluated 6-base sequencing with duet evoC, which simultaneously profiles 5mC, 5hmC, genetic variants and fragmentomics features from a single cfDNA sample.
