When working with cfDNA, every fragment has the potential to contribute valuable biological insight. However, conventional library preparation methods can alter methylation signals and fragment characteristics before they are measured, limiting sensitivity for liquid biopsy applications and force you to choose between genetics, epigenetics and fragmentomics.
We will discuss how a single-stranded ligation approach preserves the native features of cfDNA by eliminating end-repair. We’ll present new data showing how improved fragment-level accuracy promises to enhance the detection of tumor-derived DNA and support more accurate tissue-of-origin analysis, helping researchers get more insight from every sample.
What you’ll learn
• Why conventional library preparation methods can alter the native biological information carried in cfDNA fragments before it is measured.
• Why preserving native cfDNA characteristics matters for sensitive liquid biopsy applications.
• How duet mosaic accurately captures multiple complementary biological signals from the same sample.
• How better quality and more complete data helps researchers generate more insight from every fragment without choosing between genetic, epigenetic, and fragmentomic information.
